CSIR-UGC NET

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Methods in Biology

Chapters

Gene Cloning and DNA Analysis

Cloning Vectors: Plasmids

Purification of DNA

Gene Transfer Methods

Cloning Vectors for E. Coli

Cloning Vectors for Eukaryotes

Cloning a Specific Gene

Polymerase Chain Reaction

Sequencing Genes and Genomes

Gene Expression & Function

Studying Genomes

Protein Production: Cloned Genes

Gene Cloning in Medicine

Gene Cloning in Agriculture

Gene Cloning in Forensic Science

Courses
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1
Gene Cloning and DNA Analysis
Notes

Available on android app

Lessons

Isolation, Classification, and Purification of DNA & RNA

1 hr 31 mins

locked
Labeling: DNA, RNA, Nucleic Acids and Methods

1 hr 28 mins

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DNA Polymerase, End Modification Enzymes, Ligase, and Nuclease

1 hr 31 mins

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Restriction Enzymes: Nomenclature, Restriction Sites, and Classification

1 hr 30 mins

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Methods in Biology: Doubt Clearing - I

1 hr 27 mins

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Restriction Enzyme & Frequency, Digestion, Mapping and Labeling

1 hr 30 mins

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Restriction Mapping & its Types with PYQs

1 hr 30 mins

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Gene Cloning: Requirements & Steps

1 hr 36 mins

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Gene Cloning, Marker and Vectors

1 hr 30 mins

locked
2
Cloning Vectors: Plasmids
Notes

Available on android app

Lessons

Restriction Enzyme, Plasmid Incompatibility, Cloning Vector & its Properties, and Screening of Recombinant

1 hr 33 mins

locked
Vectors: Restriction Site, Host Cell, Plasmids, and Plasmid

1 hr 30 mins

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Biology: PYQs - I

1 hr 33 mins

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Vectors: Host, Cloning and Bacteriophage

1 hr 32 mins

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Vectors: Hybrid Plasmid, Phagemid & Zyplasmid, Prokaryotic and Cloning

1 hr 34 mins

locked
3
Purification of DNA

Lessons

Process of Agrobacterium, Cloning, Isolation & Purification of DNA

1 hr 11 mins

locked
Isolation & Purification of RNA, Components, and Variant of PCR

1 hr 9 mins

locked
4
Gene Transfer Methods
Notes

Available on android app

Practice

Lessons

Types of Viruses, T-DNA, Gene Transfer and Agrobacterium

1 hr 30 mins

locked
DNA, Gene Expression, Cloning, and Enzymes: Problem Solving

1 hr 33 mins

locked
5
Cloning Vectors for E. Coli
Notes

Available on android app

Lessons

HFR Strain, Escherichia Coli Chromosome and Microbial Genetic Mapping

1 hr 35 mins

locked
6
Cloning Vectors for Eukaryotes
Notes

Available on android app

Lessons

Gene Cloning, Gene Isolation & DNA Polymerase

1 hr 10 mins

locked
Vectors, Enzymes and Classification of Enzymes

1 hr 14 mins

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Classification of Restriction Enzymes

1 hr 2 mins

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Vectors Properties & Plasmid

1 hr 13 mins

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Artificial Chromosomes, Lambda DNA & Phages

52 mins

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Agrobacterium: Ti Plasmid, T-DNA Transfer, RB & Virulence Gene (ViR)

1 hr 22 mins

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VIR, T-DNA Mechanism of Transfer and Integration

1 hr 9 mins

locked
7
Cloning a Specific Gene
Notes

Available on android app

Lessons

Gene Cloning: Vector & Enzymes

1 hr 31 mins

locked
Vector, Enzymes & Problem Solving

1 hr 29 mins

locked
Gene Cloning: Problem Solving Session

1 hr 34 mins

locked
Alpha Complementation Test, Expressive Vector & its Components

1 hr 27 mins

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Components of Expressive, Shutter, Viral Vector & Restrictive Enzymes

1 hr 29 mins

locked
8
Polymerase Chain Reaction
Notes

Available on android app

Lessons

Polymerase chain reaction (PCR): Modification, Steps & Reactions

1 hr 29 mins

locked
Taq man Probes, Nested, RT & Hot Start PCR, Colony & Multiplex

1 hr 30 mins

locked
9
Sequencing Genes and Genomes
Notes

Available on android app

Lessons

Vectors, DNA Sequences, Transcript Analysis and Chain Termination

1 hr 34 mins

locked
Biotechnology: Chemical Degradation, PCR, and RTPCR

1 hr 26 mins

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Biotechnology: Gel Retardation, DNA Footprinting and Modification

1 hr 33 mins

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DNA & Gene: PYQs Solving

1 hr 33 mins

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Gene Library, Genomic, Blotting Techniques, and Blotting Membrane

1 hr 33 mins

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Types of Blotting, Hybridisation and Electrophoresis

1 hr 35 mins

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Electrophoresis, SDS - PAGE and DNA Fingerprinting

1 hr 34 mins

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Prokaryotic, DNA and Gene: PYQs

1 hr 32 mins

locked
10
Gene Expression & Function
Notes

Available on android app

Lessons

Gene Expression Study, cDNA Microarray, and Problem Solving

47 mins

locked
RNA Sequencing, Northern Blotting, cDNA, and Protein Microarray

43 mins

locked
Gene Expression Study & Markers: SDS Page Set-Up, Western Blotting, Types, and Polymorphism

59 mins

locked
Molecular Marker (AFLP & RAPD) & Gene Library Construction

1 hr 3 mins

locked
Screening of Clones, cDNA Library Construction, and Shotgun Sequencing

57 mins

locked
11
Studying Genomes
Notes

Available on android app

Lessons

Genome Library & cDNA and Doubt Clearing on Blotting

1 hr 28 mins

locked
Genome Library and Vector: Revision & PYQs

1 hr 36 mins

locked
12
Protein Production: Cloned Genes
Notes

Available on android app

Lessons

Protein Structure: Bonds, Function, Proline, Peptide & Ramachandran Plot

1 hr 33 mins

locked
Fibrous and Globular Protein: Collagen, Elastin, Biosynthesis, Keratin & Myoglobin

1 hr 33 mins

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Myoglobin, Haemoglobin, Kinetic Hameoglobin, PH, BPG & Sickle Haemoglobin

1 hr 32 mins

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Protein Structure: Primary, Secondary & Tertiary

1 hr 31 mins

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Protein Solubility: Introduction, Factors, PH & Temperature

1 hr 30 mins

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Enzymes, Kinetics: Introduction, Classification, Mechanism, Catalyzed & Uncatalyzed

1 hr 34 mins

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Protein Folding: Introduction, Degradation, Prions & Mice Model

1 hr 33 mins

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Enzymes Inhibition Kinetics: Introduction, Approach, Model, KM & VMAX

1 hr 35 mins

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Protein Analysis: Introduction, Sanger’s Method & Hydrolysis

1 hr 32 mins

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Protein: Bonds, Denaturation, Mechanism, Enzymes & Allosteric Modulation

1 hr 33 mins

locked
13
Gene Cloning in Medicine
Notes

Available on android app

Lessons

Transgenics for Quality Improvement, Agrobacterium & Gene Therapy

1 hr 30 mins

locked
Biology: Vector in Gene Therapy, Assistance Therapy & RNA Interference

1 hr 34 mins

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Microbial Fermentations

1 hr 32 mins

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Bacteria & Structure

1 hr 29 mins

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Biosynthesis of Peptidoglycan, Gram Staining, Bacterial & Endospores

1 hr 29 mins

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Classification of Viruses, Lysogenic & Lytic Development and Bioremediation

1 hr 29 mins

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Problem Solving Session

1 hr 31 mins

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Bioremediation, Ex Situ Technologies & Phytoremediation

1 hr 30 mins

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Vaccines: Types, Advantages, Disadvantages & Live-Attenuated Vaccine

1 hr 30 mins

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Types & Vaccine Derived Polioviruses

1 hr 34 mins

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Herd Immunity & Problem Solving

1 hr 25 mins

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Marker: Introduction, Characteristics & DNA Hybridization

1 hr 30 mins

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Markers Based on PCR Amplifications

1 hr 29 mins

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Somatic Hybridization, Vaccine & Problem Solving

1 hr 32 mins

locked
14
Gene Cloning in Agriculture
Notes

Available on android app

Lessons

Methods of Cell Culture, Passaging Cells, Cryopreservation, and Plant Tissue Culture

1 hr 30 mins

locked
Somatic Hybridization, Hairy Root Culture, Transgenic Technology, and Modern Plant Breeding

1 hr 29 mins

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Gene Transfer & Breeding Methods

1 hr 29 mins

locked
15
Gene Cloning in Forensic Science
Notes

Available on android app

Lessons

Polymer Chain Reaction & Problem Solving

1 hr 31 mins

locked
Gene Knockout: Reverse Transcript, Vector & Reverse Transcription

1 hr 31 mins

locked
CRISPR: Introduction & Doubt Clearing

1 hr 27 mins

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Biology: Problem Solving - I

1 hr 36 mins

locked
DNA Sequencing, Sanger Sequencing & Pyrosequencing

1 hr 36 mins

locked
Next Generation DNA Sequencing, Microarray & Its Principle

1 hr 40 mins

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Molecular Marker: Introduction, Classification & Microsatellite

1 hr 32 mins

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Biology: Problem Solving - II

1 hr 27 mins

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DNA Fingerprinting: Rapid Amplification of cDNA Ends

1 hr 35 mins

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Antibody Generation: Introduction, Hybridoma Technique & Native Page

1 hr 29 mins

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ELISA: Concept & Its Classification

1 hr 35 mins

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Data Analysis, Chromatin Immunoprecipitation Assay & Problem Solving

1 hr 33 mins

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Flow Cytometry, Flowers Cane Activated Cell Sorting & Problem Solving

1 hr 26 mins

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Spectroscopy, Electromagnetic Radiation & Types

1 hr 24 mins

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X Ray Diffraction: Chromatography & Problem Solving

1 hr 27 mins

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Mass Spectroscopy, Velocity Selector, Magnetic Field & Protein Sequencing

1 hr 28 mins

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Microscopy: Microscope & Its Types

1 hr 23 mins

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Revision of Techniques & Crystallography

1 hr 16 mins

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NMR Spectroscopy, Chemical Shift, Splitting & Peak Integration

1 hr 22 mins

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Phase Contrast, Confocal Microscope & Problem Solving

1 hr 33 mins

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Scanning & Transmission Electron Microscope, and Problem Solving

1 hr 24 mins

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Electron Transport Chain, Gluconeogenesis & Protein

1 hr 33 mins

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Terminal Transfer Enzyme, Alpha-Complementation Test & Adaptors

1 hr 7 mins

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